Chapters of THO are student-led organizations run by campus captain ambassadors. Their main goal is to extend The Hidden Opponent’s mission to their respected campus and athletic program. Below is a list of chapters associated with The Hidden Opponent.
| American University | Washington, D.C. | Anne Marie Barbour |
| Arcardia University | Glenside, Pennsylvania | Anna Intartaglia, Abbey Finkill |
| Bryn Mawr College | Bryn Mawr, PA | Sidney Bachrach, Angela Khoudary |
| Coe College | Cedar Rapids, IA | Dexter Hein-Harrison, Jaeden Peterson |
| Carson-Newman University | Jefferson City, Tennessee | Emilee Futrell, Victoria Hunter |
| Concordia University St. Paul | Saint Paul, Minnesota | Sarah Golemme |
| Connecticut College | New London, Connecticut | Abbi King |
| Denison University | Granville, Ohio | Delia Crotty, Annie Rau |
| Eastern Illinois University | Charleston, Illinois | Sabrina Chu |
| Eastern Michigan University | Ypsilanti, MI | Abbey Kearney |
| Fairmont State University | Fairmont, West Virginia | Kelsey Moeller, Kaitlyn Stumpf |
| Franklin Pierce University | Rindge, New Hampshire | Cullen Johnson, Mads Leslie |
| George Washington University | Washington, D.C. | Maddie Loder |
| Hobart and William Smith Colleges | Geneva, New York | Kristen McCormick |
| Indiana University | Bloomington, Indiana | Margaret Rogers, Jayna Dolfi |
| Ithaca College | Ithaca, New York | Arla Davis |
| Johns Hopkins University | Baltimore, Maryland | Grace Van Atta, Jenny Zwerling |
| Long Island University | Brookville, New York | Megan Bouveur, Caitlyn Kanemitsu |
| Loyola University Maryland | Baltimore, Maryland | Lauren Joyce, Corinne Filograna |
| McDaniel College | Westminster, Maryland | Kaylen Buschhorn |
| Minot State University | Minot, ND | Calli Delsman |
| Mississippi State University | Starkville, Mississippi | |
| Morehead State University | Morehead, Kentucky | Michelle Jerantowski |
| North Park University | Chicago, Illinois | Hailey Bianco |
| Shenandoah University | Winchester, Virginia | Patrick Maneval, Maddie Re |
| Southern New Hampshire University | Manchester, New Hampshire | Helene Servais |
| Sweet Briar College | Sweet Briar, Virginia | June Alomari |
| The College of New Jersey | Ewing, NJ | Lauren Cammarata, Carolyn Mazzacco |
| University of Hartford | West Hartford, Connecticut | Julianna Kocenski |
| University of Kentucky | Lexington, Kentucky | Olivia Huffman |
| University of Lynchburg | Lynchburg, Virginia | |
| Ursinus College | Collegeville, Pennsylvania | Skylar Knapp |
| Wagner College | Staten Island, New York | Natalie Morse |
| Washington and Lee University | Lexington, Virginia | Jordan Diehl |
| West Virginia Wesleyan College | Buckhannon, West Virginia | Alyssa Serrano |
